Low Temperature Freezer FAQ Guide: Must-Know Tips For Laboratory Or Hospital
Adding a low temperature freezer to your lab or hospital is a necessary investment that can change two important connections to cryobiology in the storage field: sample and temperature. Our laboratory freezer FAQ Guide provides insights and answers to help you make an informed decision. Designed for researchers in studies, laboratories, hospitals, clinics, and more, this guide clearly explains frequently asked questions about lab freezer.
It covers how ultra low temperature freezer work, temperature and capacity ranges, and more. The guide also covers key issues such as selection requirements and how to buy. For researchers who focus on energy-efficient and temperature-controlled ult freezer, the guide focuses on how samples are stored and how temperatures are selected.
With the growing popularity of medical refrigerator in the low-temperature biomedical field, this guide discusses how to use lab refrigerator for storing pharmaceuticals, blood, cells, vaccines, and more. Whether you're starting from scratch or looking to enhance the conditions in which your samples are stored, this guide provides you with all the information you need to make a successful selection.
What Is An Low Temperature Freezer?
Low temperature refrigerator, also known as ultra-low temperature freezer, ultra-low temperature preservation box, deep low temperature refrigerator. It is suitable for low temperature storage of tuna fish, electronic devices, special materials and preservation of plasma, biological materials, lean seedlings, reagents, biological products, chemical reagents, strains, biological samples and other low temperature preservation.
What Is The Features Of Low Temperature Freezer?
Extremely low temperature: It can reach minus 164 degrees Celsius, which can be used for preserving biological samples, special materials and so on.
Stable performance: It can keep the low temperature for a long time to ensure the activity and safety of the items.
Precise temperature control: Can precisely control the temperature to meet the storage needs of different samples.
What Is Low Temperature Freezer Used For?
Ultra low freezers are widely used in the fields of medicine, biological sciences, drug research and development, and environmental sciences, etc. They can maintain high quality and long-term preservation of samples and products, and provide strong support for scientific research and medical diagnosis.
What Are The Types Of Low Temperature Freezer?
Precautions For The Use Of Low Temperature Refrigerator.
After the first installation, make sure that the cryo freezer is fixed will not be moved casually.
Ensure good ventilation, reserved space for heat dissipation on both sides and back of the refrigerator, and stable power voltage.
After installing the deep fridge freezer, don't plug it in directly to avoid damage, wait for the compressor and refrigerant to rest for 4-6 hours before plugging it in.
After powering on and setting the target temperature, don't rush to store the samples, first let the machine run for more than 24 hours with no load, make sure the temperature is stable before putting in the samples.
When there are more samples, they should be stored in batches, not full at one time.
Open the door time and frequency should be reduced, use trays or freezing boxes to centralize access to reduce the loss of cold air.
Use the freezing shelf to store in layers to ensure that the cold air circulation channel is smooth.
Defrost and clean regularly.
Handle power failure, close the medicine refrigerator door immediately to reduce the escape of cold air, or activate the backup power.
Abnormal temperature alarm.
Wear anti-freezing gloves during operation to avoid frostbite caused by direct contact with low temperature surfaces.
Waste refrigerant needs to be handled according to environmental protection regulations and direct discharge is prohibited.
What Is The Maintenance And Care Of Low Temperature Freezer?
The maintenance and upkeep of the ultra low temp freezer is especially important to prolong its life and normal use. Inaccurate temperature control often leads to damage to the preserved objects, which greatly affects the experimental results and thus the normal conduct of the research work.
Refrigerator Cleaning
Clean the laboratory refrigerators once a month to ensure its cleanliness.
Remove a small amount of dust from the inside and outside of the deep freezer refrigerator and accessories with a dry cloth, or use a neutral detergent if the lab refrigerators are too dirty, and then rinse thoroughly with purified water after cleaning. However, do not rinse inside and on the upper part of the refrigerator as this may damage the insulation and cause malfunction.
The compressor and other mechanical parts do not need to be lubricated. Be careful when cleaning the love fan at the rear of the compressor.
After cleaning, perform a safety check to ensure that the pharmaceutical refrigerator is properly plugged in and not connected inappropriately;
Ensure that the plug is not abnormally hot;
Ensure that the power and distribution cords on the back of the medication fridges are not cracked or scored.
Condenser filter cleaning
Clean the condenser surface (located on the back or bottom) monthly with a vacuum cleaner or soft bristle brush.
If the environment is dusty, increase the cleaning frequency to every two weeks.
Defrosting
To defrost automatically or manually, gently scrape the ice with a plastic spatula; do not use metal tools or hammering as this may damage the interior walls. Wipe up standing water with an absorbent cloth and dry thoroughly before restarting.
What Is The Application Of Low Temperature Freezer?
Laboratory: Preserve biological samples such as cells, tissues, plasma and strains to ensure activity and stability. They not only maintain the integrity and stability of samples during long-term refrigeration, but also prevent changes in enzymes, metabolites and other organic molecules in cell and tissue samples, making them widely used in many basic research and clinical applications.
Pharmaceuticals & Biologics: Storage of vaccines and high-value biologics to maintain potency and activity.
Food & Beverage: Flash-freeze high-end ingredients, such as tripe, to maintain freshness, flavor and nutrition, and extend shelf life.
Scientific research field: Preserve biological samples, such as cells, tissues, plasma, strains, to prevent sample degradation and ice crystal formation.
Medical field: Storage of vaccines and medicines to ensure their activity and effectiveness
Biomaterials: For preserving and testing high-temperature superconductors, semiconductors and other materials. Used for aging of metals to enhance material stability and strength, commonly used for bearings and precision parts processing.
Chemical field: Storage of chemical reagents to prevent decomposition or deterioration.
What Samples Can Be Stored In An Ult Freezer And How To Store?
What should I be aware of when freezing and accessing samples?
Cryopreservation process
Short-term storage: -40℃ (applicable to some DNA, protein, but not more than 1 month).
Long-term storage: -86℃ ultra-low temperature refrigerator or liquid nitrogen gas phase (-150℃~-196℃), applicable to cells, RNA and other easily degradable samples.
Freezing equipment needs to be equipped with temperature monitoring and alarm system, and the status of liquid nitrogen reserve or refrigerator compressor should be checked regularly.
Sample use
Cells/living samples: thaw quickly in a 37℃ water bath, remove the protective agent immediately after thawing.
Nucleic acids/proteins: can be thawed slowly at 4°C or operated on ice to reduce the risk of degradation.
Avoid repeated freezing and thawing
If you need to split the sample after thawing, it is recommended to split it into smaller volumes instead of freezing and thawing the same tube several times.
What Is The Refrigeration Working Principle Of Low Temperature Freezer?
The principle of medical refrigerators is to slow down or stop biochemical reactions and microbial growth by lowering the temperature, so as to maintain the stability and activity of samples or products. Specifically, a cryogenic refrigerator can reduce the temperature to -80C or lower, and this low temperature can effectively slow down or prevent the occurrence of biochemical reactions such as cell division, enzyme activity, biomolecule explanation, protein inactivation and so on. Therefore, cryogenic refrigerators are often used to store biological samples, vaccines, serums, enzymes, antibodies, DNA and RNA and other biological products, as well as drugs and chemicals.
What Is The Temperature Range Of A Low Temperature Freezer?
The temperature range of so-low ultra low freezer is from -40°C to -164°C: the main applications are the long-term preservation of cells, skin tissues, DNA/RNA, vaccines, pharmaceuticals, scientific research, cryogenic testing of special materials, bones, sperms etc.
What's The Capacity Of An Low Temperature Freezer?
Cialan’s low temperature freezer capacity is from 60L to 1000L. It can meet the different needs of users.
Common Problems And Solutions Of Low Temperature Refrigerator.
Temperature continues to rise
May be refrigerant leakage, compressor failure. Please contact the manufacturer for after-sales service, disassembly by yourself is prohibited.
Insufficient cooling of medical fridge
(1) Check whether there is frost on the surface of the evaporator;
(2) Whether the refrigerator door is opened and closed too often;
(3) Whether the back of the refrigerator is in contact with the wall;
(4) Whether too many items are put in.
Refrigerator is too noisy
(1) Check if the base plate is solid;
(2) If the medical freezer is stable; if not, adjust the movable screws to support the four fishes firmly on the bottom;
(3) Check if there are objects touching the back of the medical freezer.
Refrigerant deficiency
If the cooling effect is poor, the refrigerator does not stop, the heat pipe does not get hot, and there is a very small airflow sound from the evaporator, these are due to a serious refrigerant deficiency caused by slow leakage.
What are the causes and solutions of high temperature alarms generated by low temperature refrigerators?
How To Use a Medical Cryogenic Refrigerator Correctly In The Summer ?
Ventilation and cooling: Ensure that there is at least 30 centimeters of cooling space around the cryo freeze machine, away from heat sources or direct sunlight, and that air conditioners or fans can be added to assist in cooling.
Room temperature suggestion: Try to place the equipment in a room below 25℃ to avoid overloading the compressor due to high temperature.
Reduce the frequency of opening the door: Plan ahead to access items to avoid frequent opening of the door leading to temperature fluctuations.
Check the sealing: Clean the door seals regularly. If aging or icing is found to cause cold leakage, replace or de-ice them in time.
Clean condenser filters: Clean them once a month to ensure cooling efficiency.
Check fan operation: Make sure the cooling fan is operating properly to avoid overheating and shutdown.
Avoid blocking the air vents: keep the items away from the air vents to ensure smooth circulation of cold air.
Control the loading capacity: Store no more than 70% of the maximum capacity to avoid affecting the cooling effect.
Enable alarm function: Turn on the high temperature alarm or remote monitoring to find the abnormality in time.
Regular defrosting: timely treatment when the frost exceeds 5mm to reduce energy consumption.
Power protection: Equipped with voltage regulator or UPS to prevent unstable voltage or power outage from affecting the equipment.
How To Use An Low Temperature Freezer For Cell Storage?
Observe the cells first, prepare a 15ml centrifuge tube, add PDS to wash off the culture medium, discard PDS, add 2ml trypsin, observe the cell digestion with a microscope to see if the cells become rounded, add 2ml complete culture medium to terminate the trypsin digestion, and blow the cells down from the bottom of the dish.
Transfer the cells into centrifuge tube for centrifugation, take out 2ml cell cryovial, record the cell name and freezing date, discard the supernatant, and then add 90% complete culture medium, add 10% DMSO, blow well.
The cells were put into 2ml cell cryovial and temporarily stored in a 4C lockable medical fridge. When leaving the cell room, remove the cell cryovial, then put the cell cryovial into the cell cryobox, and put the cell cryobox into the -86C medical deep freezer for 24 hours. The next day take the liquid nitrogen, pour the liquid nitrogen into the foam box, cover the foam lid as soon as possible, put the cells into the cryobox, and put the cryobox into the foam box.
Take out the corresponding cryobox from the liquid nitrogen tank, put on the protective tools, take out the cryobox from the cryo rack of the liquid nitrogen tank, put the cryovials into the cryobox according to the number, then put the cryobox back to the cryo rack of the liquid nitrogen tank, lock the locking button, and put the cells into the liquid nitrogen tank for freezing.
Which Drugs/Medicines Are Stored In The Low Temperature Freezer?
Ambient (15-25°C): Most oral tablets, capsules, topical drugs, etc.
Refrigerated (2-8℃): Suppositories, insulin, vaccines, biological preparations, proprietary Chinese medicines, human albumin.
Frozen (below -20℃): Some long-acting drugs or special biological products (e.g. certain monoclonal antibody drugs).
Keep away from light: Photosensitive drugs (e.g. nitroglycerin, vitamin B12) need to be stored in original brown bottles or light-proof boxes.
What Temperature Should a Freezer Be For Medicine?
Ambient storage of medicines means storing and keeping medicines at a temperature of 10-30°C, refrigerated storage of medicines means storing and keeping medicines at a temperature of 2-10°C, and cool storage of medicines means storing and keeping medicines at a temperature not exceeding 20°C.
How To Store Blood By An Low Temperature Freezer?
Whole blood: Stored at 2-8C, periodic mixing is required to prevent stratification.
Plasma: -25C for freezing and preservation. Quick freeze after dispensing, avoid repeated freezing and thawing.
Serum: Keep frozen at -20C, centrifuge and remove clarified liquid.
Platelets: Stored at 22C with oscillation to prevent aggregation failure and maintain platelet activity and integrity.
Red blood cells: 2-8C liquid preservation to avoid hemolysis and regular color checks. 2-8℃ blood refrigerator is a specialized device for storing blood and blood products.
What Temperature Should a Medical Refrigerator Be For Vaccines?
Vaccines are made of inactivated pathogenic microorganisms or inactivated pathogenic microorganisms or their metabolites, and most of their components are proteins, which are generally afraid of heat and light, because light and heat can lead to protein denaturation, and once a live vaccine is inactivated, it loses its original immune effect, and inactivated vaccines do not produce an immune effect when they are inoculated. Therefore, vaccines need to be kept under refrigeration throughout the preservation, and individual vaccines need to be frozen.
Inactivated vaccines should be stored at 2~8℃ vaccine refrigerator, avoid freezing or high temperature.
Live vaccines should be stored in ultra-low temperature below -15℃vaccine refrigerator , and should be used within the specified time after thawing.
Can Plasma Be Stored In Hospital Refrigerator?
Yes.
Plasma: -25C for freezing and preservation. Quick freeze after dispensing, avoid repeated freezing and thawing.
How To Defrost An Low Temperature Freezer?
Some of Cialan models are auto defrost deep freezer and do not require manual defrosting.
Some models require manual defrosting. Use a plastic scraper to gently remove loose ice, do not scrape the interior walls. For fast defrosting, use a hair dryer (cold air mode) or fan as an aid.
How Much Is An Low Temperature Freezer?
It wouldn’t be right to mention one fixed amount.
The ultra low freezer price will vary from model to model.
To know the exact price, you can ask your preferred manufacturer to provide a quotation for the type of ultra low lab freezer you want.
Upright Low Temperature Freezer Vs Chest Low Temperature Freezer
Medical Deep Freezer Vs Home Freezer
What Is The Normal Temperature Of a Laboratory Refrigerator?
-25C general cold refrigerator: Used for storing reagents, samples, etc. that do not require high temperatures.
-40C low-temperature refrigerator: Can reach a lower temperature, suitable for storing temperature-sensitive samples.
Explosion-proof refrigerator: Used to store flammable, explosive or corrosive substances
-86C to -164C Ultra-low Temperature Refrigerator: Can reach very low temperature, such as below -86℃ for the storage of special samples.
2-8C blood refrigerator: Specialized for storing blood products.
What Is The Temperature Range For Pharmacy Refrigerator?
Designed with the unique needs of the pharmacy environment in mind, the Medical Grade Pharmacy Refrigerator safely stores vaccines, pharmaceuticals, chemotherapy drugs and other products that require safe refrigeration. It does an excellent job of controlling the temperature inside the cabinet within a range of 2°C to 8°C. We design our pharmaceutical coolers with high brightness digital temperature to ensure that the display temperature is accurate to 0.1℃.
How To Buy An Low Temperature Freezer From China?
Importing high performance cryogenic ultra low freezer from China can be difficult for those who are new to international imports.
Purchasing from a reputable manufacturer and supplier eliminates hassle of logistics, as they handle the safe and reliable delivery of the product directly to your place.
When buying the pharmacy refrigerators, it is important to keep the following points in mind:
1. Confirm that your selected supplier can securely export and deliver the machine, and ensure that your agreement includes all essential shipping information.
2. Choose the delivery way (by sea, air, or express) based on your preference.
3. Remember to factor in duty imports and taxes when calculating the total cost.
How Does a Manufacturer Deliver The Low Temperature Freezer?
Safe shipping is so important as quality manufacturing.
A responsible manufacturer should deliver your pharmaceutical grade refrigerator safely.
Most lab freezers and refrigerators manufacturers use export wooden case to pack the device.
You should read all the necessary instructions available on the sides of the boxes before boxing.
Is There Any Warranty On Low Temperature Freezer?
For optimal performance, it is important to regularly maintain your so low ultra low freezer, so it can operate efficiently for an extended period. It is essential to be aware of the warranty coverage for your equipment, as it ensures that any manufacturing issues will be repaired at no additional cost by the supplier. Therefore, it is recommended to purchase an ultra low temperature refrigerator from a manufacturer that offers warranty services —Cialan.
Reputable refrigerador de laboratorio manufacturers typically provide a 1-year warranty. for example, offers complimentary repair and maintenance services during the first year.
Before purchasing an ultra low temperature deep freezer, it is advisable to review the warranty period and other associated terms and conditions.
What After-Sale Service Does A Low Temperature Freezer?
The reliable china laboratory refrigerator freezer combination suppliers offers the following after-sale services:
1. Secure product delivery.
2. Thorough inspection prior to installation.
3. Complimentary online guidance for device installation and maintenance.
4. Free training for your users.
5. Regular assessments of your pharmaceutical refrigerator with freezer.
Can You Do OEM&ODM For Low Temperature Freezer?
Yes, Cialan can do OEM#OEM for our users, just send us your requirements for ult lab freezer.
Where To Find Low Temperature Freezer Manufacturers?
Numerous online directories can connect you with pharmacy grade refrigerator manufacturers.
A simple search for "top-rated low temperature deep freezer manufacturers" yields multiple options. However, it's essential to exercise caution when selecting a manufacturer from online search results. Before making a decision, conduct thorough research on the company's background and reputation. Look up reviews on “Google” and reputable review sites to gauge their credibility.
Additionally, It's also crucial to review their portfolio of request that potential manufacturers share examples of their previous work to ensure they meet your standards.
How To Choose A Right Low Temperature Freezer?
Temperature range: Determine the required temperature range according to the requirements of the stored items.
Capacity size: select the appropriate capacity based on the needs of the sample.
Stability: Ensure that the refrigerator can maintain the required temperature stably.
Explosion-proof performance: If necessary, choose a refrigerator with explosion-proof function.
Quality and reliability: Choose a reliable brand and product.
Energy consumption and noise: Consider energy consumption and noise level during operation.
What Should You Look For In The Best Low Temperature Freezer?
The best ultra low temperature freezer needs precise and stable temperature control, efficient cooling system, energy saving, environmental protection, durability, easy maintenance and safety.
It covers how ultra low temperature freezer work, temperature and capacity ranges, and more. The guide also covers key issues such as selection requirements and how to buy. For researchers who focus on energy-efficient and temperature-controlled ult freezer, the guide focuses on how samples are stored and how temperatures are selected.
With the growing popularity of medical refrigerator in the low-temperature biomedical field, this guide discusses how to use lab refrigerator for storing pharmaceuticals, blood, cells, vaccines, and more. Whether you're starting from scratch or looking to enhance the conditions in which your samples are stored, this guide provides you with all the information you need to make a successful selection.
What Is An Low Temperature Freezer?
Low temperature refrigerator, also known as ultra-low temperature freezer, ultra-low temperature preservation box, deep low temperature refrigerator. It is suitable for low temperature storage of tuna fish, electronic devices, special materials and preservation of plasma, biological materials, lean seedlings, reagents, biological products, chemical reagents, strains, biological samples and other low temperature preservation.
What Is The Features Of Low Temperature Freezer?
Extremely low temperature: It can reach minus 164 degrees Celsius, which can be used for preserving biological samples, special materials and so on.
Stable performance: It can keep the low temperature for a long time to ensure the activity and safety of the items.
Precise temperature control: Can precisely control the temperature to meet the storage needs of different samples.
What Is Low Temperature Freezer Used For?
Ultra low freezers are widely used in the fields of medicine, biological sciences, drug research and development, and environmental sciences, etc. They can maintain high quality and long-term preservation of samples and products, and provide strong support for scientific research and medical diagnosis.
What Are The Types Of Low Temperature Freezer?
| Temperature range | 2℃~8℃ pharmacy refrigerator 4℃ blood bank storage refrigerator -22 ℃ platelet incubator -25 ℃ biomedical freezer -40 ℃-60 ℃ low temperature refrigerator -86 ℃ ultra-low temperature refrigerator -150 ° C/-164 ° C ultra-low temperature refrigerator |
| Capacity | 60L-1000L |
| Application | Medical low temperature refrigerator Laboratory low temperature refrigerator Industrial low temperature refrigerator Home low temperaturerefrigerator |
| Structure | Upright low temperature refrigerator Chest low temperature refrigerator Portable medical fridge Undercounter ultra low freezer Transportation low temperature refrigerator Explosion proof low temperature refrigerator |
Precautions For The Use Of Low Temperature Refrigerator.
After the first installation, make sure that the cryo freezer is fixed will not be moved casually.
Ensure good ventilation, reserved space for heat dissipation on both sides and back of the refrigerator, and stable power voltage.
After installing the deep fridge freezer, don't plug it in directly to avoid damage, wait for the compressor and refrigerant to rest for 4-6 hours before plugging it in.
After powering on and setting the target temperature, don't rush to store the samples, first let the machine run for more than 24 hours with no load, make sure the temperature is stable before putting in the samples.
When there are more samples, they should be stored in batches, not full at one time.
Open the door time and frequency should be reduced, use trays or freezing boxes to centralize access to reduce the loss of cold air.
Use the freezing shelf to store in layers to ensure that the cold air circulation channel is smooth.
Defrost and clean regularly.
Handle power failure, close the medicine refrigerator door immediately to reduce the escape of cold air, or activate the backup power.
Abnormal temperature alarm.
Wear anti-freezing gloves during operation to avoid frostbite caused by direct contact with low temperature surfaces.
Waste refrigerant needs to be handled according to environmental protection regulations and direct discharge is prohibited.
What Is The Maintenance And Care Of Low Temperature Freezer?
The maintenance and upkeep of the ultra low temp freezer is especially important to prolong its life and normal use. Inaccurate temperature control often leads to damage to the preserved objects, which greatly affects the experimental results and thus the normal conduct of the research work.
Refrigerator Cleaning
Clean the laboratory refrigerators once a month to ensure its cleanliness.
Remove a small amount of dust from the inside and outside of the deep freezer refrigerator and accessories with a dry cloth, or use a neutral detergent if the lab refrigerators are too dirty, and then rinse thoroughly with purified water after cleaning. However, do not rinse inside and on the upper part of the refrigerator as this may damage the insulation and cause malfunction.
The compressor and other mechanical parts do not need to be lubricated. Be careful when cleaning the love fan at the rear of the compressor.
After cleaning, perform a safety check to ensure that the pharmaceutical refrigerator is properly plugged in and not connected inappropriately;
Ensure that the plug is not abnormally hot;
Ensure that the power and distribution cords on the back of the medication fridges are not cracked or scored.
Condenser filter cleaning
Clean the condenser surface (located on the back or bottom) monthly with a vacuum cleaner or soft bristle brush.
If the environment is dusty, increase the cleaning frequency to every two weeks.
Defrosting
To defrost automatically or manually, gently scrape the ice with a plastic spatula; do not use metal tools or hammering as this may damage the interior walls. Wipe up standing water with an absorbent cloth and dry thoroughly before restarting.
What Is The Application Of Low Temperature Freezer?
Laboratory: Preserve biological samples such as cells, tissues, plasma and strains to ensure activity and stability. They not only maintain the integrity and stability of samples during long-term refrigeration, but also prevent changes in enzymes, metabolites and other organic molecules in cell and tissue samples, making them widely used in many basic research and clinical applications.
Pharmaceuticals & Biologics: Storage of vaccines and high-value biologics to maintain potency and activity.
Food & Beverage: Flash-freeze high-end ingredients, such as tripe, to maintain freshness, flavor and nutrition, and extend shelf life.
Scientific research field: Preserve biological samples, such as cells, tissues, plasma, strains, to prevent sample degradation and ice crystal formation.
Medical field: Storage of vaccines and medicines to ensure their activity and effectiveness
Biomaterials: For preserving and testing high-temperature superconductors, semiconductors and other materials. Used for aging of metals to enhance material stability and strength, commonly used for bearings and precision parts processing.
Chemical field: Storage of chemical reagents to prevent decomposition or deterioration.
What Samples Can Be Stored In An Ult Freezer And How To Store?
| 2℃~8℃ pharmacy refrigerator | Cold storage of biological products, reagents, etc. |
| 4℃ blood bank storage refrigerator | Blood storage. |
| -22 ℃ platelet incubator | Platelet storage. |
| -25 ℃ biomedical freezer | storage of plasma, reagents, biological materials, etc. |
| -40 ℃ low temperature refrigerator | storage of seeds, low-temperature material experiments, biological products, frozen plasma. |
| -86 ℃ ultra-low temperature refrigerator | storage of cells, tissues, test samples, vaccines, strains, plasmids, viruses, bacteria, blood erythrocytes, leukocytes, germplasm banks and other high value-added samples ultra-low-temperature storage, hardness of the material toughness of processing. |
| -150℃/-164℃ ultra-low temperature refrigerator | At this temperature, usually considered the bacterial and cellular activity critical temperature, biological activity is greatly reduced, can be preserved in the medium and long term cellular enclosure structure stability. |
| Note: If the storage of flammable and explosive reagents, remember to choose explosion-proof refrigerator. | |
What should I be aware of when freezing and accessing samples?
Cryopreservation process
Short-term storage: -40℃ (applicable to some DNA, protein, but not more than 1 month).
Long-term storage: -86℃ ultra-low temperature refrigerator or liquid nitrogen gas phase (-150℃~-196℃), applicable to cells, RNA and other easily degradable samples.
Freezing equipment needs to be equipped with temperature monitoring and alarm system, and the status of liquid nitrogen reserve or refrigerator compressor should be checked regularly.
Sample use
Cells/living samples: thaw quickly in a 37℃ water bath, remove the protective agent immediately after thawing.
Nucleic acids/proteins: can be thawed slowly at 4°C or operated on ice to reduce the risk of degradation.
Avoid repeated freezing and thawing
If you need to split the sample after thawing, it is recommended to split it into smaller volumes instead of freezing and thawing the same tube several times.
What Is The Refrigeration Working Principle Of Low Temperature Freezer?
The principle of medical refrigerators is to slow down or stop biochemical reactions and microbial growth by lowering the temperature, so as to maintain the stability and activity of samples or products. Specifically, a cryogenic refrigerator can reduce the temperature to -80C or lower, and this low temperature can effectively slow down or prevent the occurrence of biochemical reactions such as cell division, enzyme activity, biomolecule explanation, protein inactivation and so on. Therefore, cryogenic refrigerators are often used to store biological samples, vaccines, serums, enzymes, antibodies, DNA and RNA and other biological products, as well as drugs and chemicals.
What Is The Temperature Range Of A Low Temperature Freezer?
The temperature range of so-low ultra low freezer is from -40°C to -164°C: the main applications are the long-term preservation of cells, skin tissues, DNA/RNA, vaccines, pharmaceuticals, scientific research, cryogenic testing of special materials, bones, sperms etc.
What's The Capacity Of An Low Temperature Freezer?
Cialan’s low temperature freezer capacity is from 60L to 1000L. It can meet the different needs of users.
Common Problems And Solutions Of Low Temperature Refrigerator.
Temperature continues to rise
May be refrigerant leakage, compressor failure. Please contact the manufacturer for after-sales service, disassembly by yourself is prohibited.
Insufficient cooling of medical fridge
(1) Check whether there is frost on the surface of the evaporator;
(2) Whether the refrigerator door is opened and closed too often;
(3) Whether the back of the refrigerator is in contact with the wall;
(4) Whether too many items are put in.
Refrigerator is too noisy
(1) Check if the base plate is solid;
(2) If the medical freezer is stable; if not, adjust the movable screws to support the four fishes firmly on the bottom;
(3) Check if there are objects touching the back of the medical freezer.
Refrigerant deficiency
If the cooling effect is poor, the refrigerator does not stop, the heat pipe does not get hot, and there is a very small airflow sound from the evaporator, these are due to a serious refrigerant deficiency caused by slow leakage.
What are the causes and solutions of high temperature alarms generated by low temperature refrigerators?
| Causes | Problems with the refrigeration system: it is a key component of the refrigerator, and once it breaks down, it will not be able to keep the temperature inside the refrigerator at the set value, thus triggering the alarm. The ambient temperature is too high: when the ambient temperature exceeds 30C, it will cause the cold air to dissipate, which will have an effect on the refrigeration effect, thus leading to the high temperature alarm. The pharmacy refrigerator door is not closed properly, so that the cold air leakage, the refrigeration effect caused by interference, which leads to high temperature alarm. Unstable power supply and frequent tripping will lead to compressor failure and cause high temperature alarm. The condenser becomes dirty, one is because the condenser filter is dirty, the second is the condenser fan is damaged. |
| Solutions | Check the refrigeration system, once the high temperature alarm, first check the working status of the refrigeration system, if there is a fault, it should be repaired or replaced immediately, long-term continuous operation without shutdown caused by failure, shutdown for more than 48 hours to reboot can be restored. Reduce the ambient temperature, you can use air conditioning or transfer the cryo freeze chamber to a low-temperature environment to cool down. Check the closure of the biomedical freezer door, to ensure that the door is well sealed to prevent the loss of cold air. A biomedical freezer door left open is usually due to too many items inside and improperly placed frames, so place items as required, clean regularly, and close the small door before closing the large one. Detect whether the compressor is faulty, if the compressor does not work properly, you need to contact the manufacturer for repair. For dirty condenser, clean the filter regularly and replace the fan. |
How To Use a Medical Cryogenic Refrigerator Correctly In The Summer ?
Ventilation and cooling: Ensure that there is at least 30 centimeters of cooling space around the cryo freeze machine, away from heat sources or direct sunlight, and that air conditioners or fans can be added to assist in cooling.
Room temperature suggestion: Try to place the equipment in a room below 25℃ to avoid overloading the compressor due to high temperature.
Reduce the frequency of opening the door: Plan ahead to access items to avoid frequent opening of the door leading to temperature fluctuations.
Check the sealing: Clean the door seals regularly. If aging or icing is found to cause cold leakage, replace or de-ice them in time.
Clean condenser filters: Clean them once a month to ensure cooling efficiency.
Check fan operation: Make sure the cooling fan is operating properly to avoid overheating and shutdown.
Avoid blocking the air vents: keep the items away from the air vents to ensure smooth circulation of cold air.
Control the loading capacity: Store no more than 70% of the maximum capacity to avoid affecting the cooling effect.
Enable alarm function: Turn on the high temperature alarm or remote monitoring to find the abnormality in time.
Regular defrosting: timely treatment when the frost exceeds 5mm to reduce energy consumption.
Power protection: Equipped with voltage regulator or UPS to prevent unstable voltage or power outage from affecting the equipment.
How To Use An Low Temperature Freezer For Cell Storage?
Observe the cells first, prepare a 15ml centrifuge tube, add PDS to wash off the culture medium, discard PDS, add 2ml trypsin, observe the cell digestion with a microscope to see if the cells become rounded, add 2ml complete culture medium to terminate the trypsin digestion, and blow the cells down from the bottom of the dish.
Transfer the cells into centrifuge tube for centrifugation, take out 2ml cell cryovial, record the cell name and freezing date, discard the supernatant, and then add 90% complete culture medium, add 10% DMSO, blow well.
The cells were put into 2ml cell cryovial and temporarily stored in a 4C lockable medical fridge. When leaving the cell room, remove the cell cryovial, then put the cell cryovial into the cell cryobox, and put the cell cryobox into the -86C medical deep freezer for 24 hours. The next day take the liquid nitrogen, pour the liquid nitrogen into the foam box, cover the foam lid as soon as possible, put the cells into the cryobox, and put the cryobox into the foam box.
Take out the corresponding cryobox from the liquid nitrogen tank, put on the protective tools, take out the cryobox from the cryo rack of the liquid nitrogen tank, put the cryovials into the cryobox according to the number, then put the cryobox back to the cryo rack of the liquid nitrogen tank, lock the locking button, and put the cells into the liquid nitrogen tank for freezing.
Which Drugs/Medicines Are Stored In The Low Temperature Freezer?
Ambient (15-25°C): Most oral tablets, capsules, topical drugs, etc.
Refrigerated (2-8℃): Suppositories, insulin, vaccines, biological preparations, proprietary Chinese medicines, human albumin.
Frozen (below -20℃): Some long-acting drugs or special biological products (e.g. certain monoclonal antibody drugs).
Keep away from light: Photosensitive drugs (e.g. nitroglycerin, vitamin B12) need to be stored in original brown bottles or light-proof boxes.
What Temperature Should a Freezer Be For Medicine?
Ambient storage of medicines means storing and keeping medicines at a temperature of 10-30°C, refrigerated storage of medicines means storing and keeping medicines at a temperature of 2-10°C, and cool storage of medicines means storing and keeping medicines at a temperature not exceeding 20°C.
How To Store Blood By An Low Temperature Freezer?
Whole blood: Stored at 2-8C, periodic mixing is required to prevent stratification.
Plasma: -25C for freezing and preservation. Quick freeze after dispensing, avoid repeated freezing and thawing.
Serum: Keep frozen at -20C, centrifuge and remove clarified liquid.
Platelets: Stored at 22C with oscillation to prevent aggregation failure and maintain platelet activity and integrity.
Red blood cells: 2-8C liquid preservation to avoid hemolysis and regular color checks. 2-8℃ blood refrigerator is a specialized device for storing blood and blood products.
What Temperature Should a Medical Refrigerator Be For Vaccines?
Vaccines are made of inactivated pathogenic microorganisms or inactivated pathogenic microorganisms or their metabolites, and most of their components are proteins, which are generally afraid of heat and light, because light and heat can lead to protein denaturation, and once a live vaccine is inactivated, it loses its original immune effect, and inactivated vaccines do not produce an immune effect when they are inoculated. Therefore, vaccines need to be kept under refrigeration throughout the preservation, and individual vaccines need to be frozen.
Inactivated vaccines should be stored at 2~8℃ vaccine refrigerator, avoid freezing or high temperature.
Live vaccines should be stored in ultra-low temperature below -15℃vaccine refrigerator , and should be used within the specified time after thawing.
Can Plasma Be Stored In Hospital Refrigerator?
Yes.
Plasma: -25C for freezing and preservation. Quick freeze after dispensing, avoid repeated freezing and thawing.
How To Defrost An Low Temperature Freezer?
Some of Cialan models are auto defrost deep freezer and do not require manual defrosting.
Some models require manual defrosting. Use a plastic scraper to gently remove loose ice, do not scrape the interior walls. For fast defrosting, use a hair dryer (cold air mode) or fan as an aid.
How Much Is An Low Temperature Freezer?
It wouldn’t be right to mention one fixed amount.
The ultra low freezer price will vary from model to model.
To know the exact price, you can ask your preferred manufacturer to provide a quotation for the type of ultra low lab freezer you want.
Upright Low Temperature Freezer Vs Chest Low Temperature Freezer
| Upright Low Temperature Freezer | Chest Low Temperature Freezer |
| Small footprint, high space utilization, each layer is equipped with a small door, opening the door to the temperature of the box has little impact. However, the temperature stability and temperature uniformity is slightly worse, not suitable for volume or heavier samples. | Covering a large area, temperature uniformity is good, suitable for large volume or large amount of sample storage. |
Medical Deep Freezer Vs Home Freezer
| Medical Deep Freezer | Home Freezer |
| Temperature range: -40C to -164C Laboratory refrigerators are generally used for storing vaccines, medicines and chemical reagents, etc., these samples require a constant storage temperature, so the requirements for vaccines, medicines, chemical reagent storage device should be a small temperature range of "thermostat". |
Temperature range:-18C Household refrigerator is a kind of low-temperature storage equipment which adopts direct cooling method of refrigeration, generally it is one of refrigeration and freezing, sharing one compressor, and there is only one controller to regulate the temperature in the freezer, its main function is to keep food fresh for the purpose, and it does not have a high requirement on the temperature stability, therefore, the household refrigerator will have the problems of temperature fluctuation and internal temperature uniformity. |
What Is The Normal Temperature Of a Laboratory Refrigerator?
-25C general cold refrigerator: Used for storing reagents, samples, etc. that do not require high temperatures.
-40C low-temperature refrigerator: Can reach a lower temperature, suitable for storing temperature-sensitive samples.
Explosion-proof refrigerator: Used to store flammable, explosive or corrosive substances
-86C to -164C Ultra-low Temperature Refrigerator: Can reach very low temperature, such as below -86℃ for the storage of special samples.
2-8C blood refrigerator: Specialized for storing blood products.
What Is The Temperature Range For Pharmacy Refrigerator?
Designed with the unique needs of the pharmacy environment in mind, the Medical Grade Pharmacy Refrigerator safely stores vaccines, pharmaceuticals, chemotherapy drugs and other products that require safe refrigeration. It does an excellent job of controlling the temperature inside the cabinet within a range of 2°C to 8°C. We design our pharmaceutical coolers with high brightness digital temperature to ensure that the display temperature is accurate to 0.1℃.
How To Buy An Low Temperature Freezer From China?
Importing high performance cryogenic ultra low freezer from China can be difficult for those who are new to international imports.
Purchasing from a reputable manufacturer and supplier eliminates hassle of logistics, as they handle the safe and reliable delivery of the product directly to your place.
When buying the pharmacy refrigerators, it is important to keep the following points in mind:
1. Confirm that your selected supplier can securely export and deliver the machine, and ensure that your agreement includes all essential shipping information.
2. Choose the delivery way (by sea, air, or express) based on your preference.
3. Remember to factor in duty imports and taxes when calculating the total cost.
How Does a Manufacturer Deliver The Low Temperature Freezer?
Safe shipping is so important as quality manufacturing.
A responsible manufacturer should deliver your pharmaceutical grade refrigerator safely.
Most lab freezers and refrigerators manufacturers use export wooden case to pack the device.
You should read all the necessary instructions available on the sides of the boxes before boxing.
Is There Any Warranty On Low Temperature Freezer?
For optimal performance, it is important to regularly maintain your so low ultra low freezer, so it can operate efficiently for an extended period. It is essential to be aware of the warranty coverage for your equipment, as it ensures that any manufacturing issues will be repaired at no additional cost by the supplier. Therefore, it is recommended to purchase an ultra low temperature refrigerator from a manufacturer that offers warranty services —Cialan.
Reputable refrigerador de laboratorio manufacturers typically provide a 1-year warranty. for example, offers complimentary repair and maintenance services during the first year.
Before purchasing an ultra low temperature deep freezer, it is advisable to review the warranty period and other associated terms and conditions.
What After-Sale Service Does A Low Temperature Freezer?
The reliable china laboratory refrigerator freezer combination suppliers offers the following after-sale services:
1. Secure product delivery.
2. Thorough inspection prior to installation.
3. Complimentary online guidance for device installation and maintenance.
4. Free training for your users.
5. Regular assessments of your pharmaceutical refrigerator with freezer.
Can You Do OEM&ODM For Low Temperature Freezer?
Yes, Cialan can do OEM#OEM for our users, just send us your requirements for ult lab freezer.
Where To Find Low Temperature Freezer Manufacturers?
Numerous online directories can connect you with pharmacy grade refrigerator manufacturers.
A simple search for "top-rated low temperature deep freezer manufacturers" yields multiple options. However, it's essential to exercise caution when selecting a manufacturer from online search results. Before making a decision, conduct thorough research on the company's background and reputation. Look up reviews on “Google” and reputable review sites to gauge their credibility.
Additionally, It's also crucial to review their portfolio of request that potential manufacturers share examples of their previous work to ensure they meet your standards.
How To Choose A Right Low Temperature Freezer?
Temperature range: Determine the required temperature range according to the requirements of the stored items.
Capacity size: select the appropriate capacity based on the needs of the sample.
Stability: Ensure that the refrigerator can maintain the required temperature stably.
Explosion-proof performance: If necessary, choose a refrigerator with explosion-proof function.
Quality and reliability: Choose a reliable brand and product.
Energy consumption and noise: Consider energy consumption and noise level during operation.
What Should You Look For In The Best Low Temperature Freezer?
The best ultra low temperature freezer needs precise and stable temperature control, efficient cooling system, energy saving, environmental protection, durability, easy maintenance and safety.
